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Gold nanoparticle based assay for detecting kanamycin in food with high sensitivity and selectivity by Dr. Chengke Wang
 

In the process of food production, kanamycin was widely used for inhibiting the harmful bacteria, and preventing a variety of infections. However, its residue in animal-derived foods (e.g., milk, meat and eggs) may lead to different degree of damage to humans (from drug resistance to poisoning). Although HPLC, ELISA etc. methods can efficiently detect kanamycin, there are still some hindrances, such as time-consuming, high cost, tedious and difficult operation. Therefore, it is desirable and urgent to develop facial, cost-efficient and rapid assays for detecting kanamycin with favorable sensitivity and selectivity.

Recently, Wang et. al. had developed two methods to detect kanamycin in milk, pork and chicken meat, i.e., gold nanoparticle (GNP) based resonance light scattering (RLS) method, and colorimetric assay based on the catalytical ability enhancement of GNPs. The limits of detection are 1 nM and 0.1 nM, respectively.

The interest of Dr. Wang was mainly on the development of biosensor to detect harmful species in environmental, food and agricultural materials, such as mycotoxins, heavy metal ions, residues of antibiotics and pesticide, etc.

 

Fig. 1 Schematic illustration of the RLS based method for detecting kanamycin using GNPs and aptamer. The GNPs maintained dispersed with the protection of aptamer (the aptamer can adhere onto the GNPs by electrostatic interaction) even in the presence of NaCl, while after the addition of kanamycin, the aptamer can be taken away from GNPs due to the specific and more strong interactions between kanamycin and aptamer, which induced the aggregation of GNPs in the presence of NaCl, and the RLS signals were enhanced dramatically, consequently. The illustration is not drawn to scale.

 

Fig. 2 The RLS based experiment for detecting kanamycin. The RLS spectra of GNPs in the presence of different concentrations of kanamycin (a), the signal intensity at 700 nm as a function of the concentration of kanamycin. Inset of (b) shows the corresponding digital images (from left to right) of the GNPs solutions with increasing the concentration of kanamycin.

 

Fig. 3. Schematic illustration of the AuNPs (gold nanoparticle) based colorimetric method for detecting kanamycin. Kanamycin can adhere onto the GNPs, and induced the formation of gold ions (Au3+) and redox-active hydroxyl free radical (¡ñOH), which dramatically enhanced the catalytical ability of AuNPs, and amounts of colored species (oxidized TMB) increased, consequently. The illustration is not drawn to scale.

E-mail: wangck@ujs.edu.cn

http://asp1.ujs.edu.cn/sp/Js_view.asp?NewsID=380

http://spswxy.ujs.edu.cn/szdw-view.asp?ArticleID=67

 

https://scholar.google.com.hk/citations?user=Zr2kpEgAAAAJ&hl=zh-CN

 

 

(School of Food and Biological Engineering)

 

¡¼Returns¡½ Hits£º384 Date£º2016-3-22
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